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Velveteen Squares

Description: Highest quality velveteen optimizes efficiency of colony transfer.All edges are overlocked stitched to increase product life. Velveteen squares are constructed from reusable 100% cotton. Each can create up to twenty replicas from a single master plate. Black material enables colonies to be easily seen between replicas.

 

  1. Protocol for initial cleaning and sterilization of velveteen squares
    1. Remove velveteen squares from package and, before the first use, wash once in a washer and dryer.
      Note:
      • Use detergent sparingly, if at all, and only during the first wash. It is important to ensure that the detergent is completely removed and will not affect your assays.
      • Best result will be seen after the initial washing.
    2. When using a dryer, dry the velveteens until they are dry to touch but have not picked up static or started to curl.
    3. Sterilize by autoclaving using the dry/wrapped cucle.
      Note:
      • We recommend using a manila envelope instead of aluminum foil, which retains moisture. Do not use envelopes with holes. The envelopes may be used for several rounds of autoclaving until they become brittle.
        • Tape the manila envelope down the center and bottom seams with laboratory tape.
        • Place 10 velveteen squares (velveteen side facing down) on a sheet of blotting paper or piece of rigid paper slightly smaller that the envelope; place a sheet of blotting paper atop the squares; lay 10 more squares (facing down); and finally lay another sheet of blotting paper on the stack.
        • Place the stack of velveteen squares and blotting paper in the manila envelope and tape closed with a small piece of autoclave tape.
        • Autoclave on the dry cycle for 30 min at 250°C and 15 psi.
  2. Protocol for using velveteen squares
    1. Remove the "sandwich" of velveteen squares and blotting paper from the manila envelope and place on your bench with the velveteen side facing down.
    2. Set aside the topmost sheet of blotting paper. (The stack of velveteens should be facinng down.)
    3. Remove a velveteen from the stack; lay velveteen on the replica block and put the collar in place.
    4. Create up to 20 replica plates from a single master and vellveteen square; then set used velveteen aside.
      Note:
      • Best results will be seen with medium that has been allowed to air dry for two or more days. Excessive moisture may be removed from medium by drying for 30 min in a laminar hood with the cover off.
    5. Move on to the next velveteen and repeat Steps 3 and 4.
    6. When finished, place the blotting paper (sterile side down) back on top of the sstcj and place the stack back inside the manila envelope.
    7. Proceed to Section C for cleaning your velveteen squares. It is important to clean your squares the same day as they were used; otherwise they will be more difficult to rinse.

  3. Protocol for maintaining velveteen squares
    1. After use, rinse velveteen squares by laying them in a sink and spraying with house distilled water.
      Note:
      • It is important to rinse the squares the same day as use.
      • Do not soak velveeteen squares in water for expanded periods of time. Prolonged exposure to water or antifungal/ antibacterial agents will weaken the fabric and the stitching.
      • The use of antifungal or antibacterial agents is not recommended and should anly be used when necessary.
    2. Allow excess water to drip off.
    3. Hang squares to dry. A few velveteen squares may be stacked on top of one another when grying. (The squares should dry within a day.)
      Note:
      • We recommend that the velveteens be completely dry before autoclaving.
      • Do not squeeze velveteen squarws to get rid of excess water.
    4. Autoclave velveteens as described in Section A, Step 3 above.
      Note:
      • Velveteen can be used, rinsed, and autoclaved about 6-8 times before it is advisable to wash them in a washer and dryer to refluff the velveteen.
      • Velveteens can be reused up to 12 times.
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