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ECL Western Blotting Substrate
  • Higher sensitivity
  • Superior signal
  • Clear background
  • Easy to use


Product contents
Catalog# EC1808-25 EC1808-100 EC1808-500
Reagent A 25 mL 100 mL 5x100 mL
Reagent B 125 µL 500 µL 5x500 µL
Sufficient membrane 250cm² 1000cm² 5000cm²
Price $70.00 $260.00 $719.00


Storage: Store reagent A and B @ 4°C0
Western Blotting Analysis (5 seconds exposure)

ECL Western Blotting Substrate

Procedure Summary

  1. Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBS/T.
  2. Prepare 1X ECL Western Blotting Substrate by mix Reagent A and Reagent B at a 1000:5 ratio (i.e., 5mL Reagent A + 25µL Reagent B for 6 x 8 cm² membrane) and add it to the blot.
  3. Incubate Reagent with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic.
  4. Expose blot to X-ray film: XAR ALF 2025 or XAR ALF 1318


Western blot analysis of diluted HeLa cell lysates using anti- β-Actin antibody. Lane 1 contained 2µg of total protein, and four 2-fold serial dilutions were applied to lanes 2-5 at 10µL/well.

Catalog #DescriptionSize (ml) Price
EC1808-25Reagent A, Reagent B, Sufficient membrane25ml$70.00Add to Cart
EC1808-100Reagent A, Reagent B, Sufficient membrane100ml$260.00Add to Cart
EC1808-500Reagent A, Reagent B, Sufficient membrane500ml$719.00Add to Cart