Properties and Applications:
Salmon-gal (6-Chloro-3-indolyl-β-D-galactopyranoside) is an alternative chromogenic substrate for β-galactosidase. Salmon-gal is used in conjunction with IPTG for detection of β-galactosidase activity in bacterial colonies in a colometric assay to detect recombinants (white) from the non-recombinants (SALMON).
Salmon-gal is cleaved at the β-1-4 bond between galactose and the 5-bromo-4-chloro-3-indolyly part of X-gal by β-galactosidase via hydrolisis. The cleavage of Salmon-gal results in the production of a Salmon colored water insoluble pigment.
In conjunction with X-glu, salmon-gal is useful for simultaneous detection of GUS and Lac activities on the same plate.
Recommended Storage: 4C or below.
Solubility: DMSO and DMFO.
Protect from light and moisture.
Allow to warm to room temperature before opening.